Optimization of Effervescent Tablet-Assisted Dispersive Liquid-Liquid Microextraction with Central Composite Design for Preconcentration of Stimulant Drug

The extraction efficiency of stimulant drug, namely caffeine, was investigated using a 23 central composite design. values optimum condition were set at 468 ?L 1-dodecanol, 1 piece effervescent tablet, and 22 °C temperature. An enrichment factor calculated as 72 for 80 mL water sample. run time conducted in less than 6 min non-polar C18 column an isocratic mobile phase (methanol: 40:60 (v/v)) controlled flow rate min-1. A good linear response achieved the range 0.01-0.50 ?g mL-1 (R2> 0.998). Detection quantification limits 0.009 0.015 mL-1, respectively. average recoveries two spiking concentration levels within 75-105% with RSD < 2% (n = 3). Real samples beverages which contained caffeine river tested proposed method, results ranged 0.021-0.56 mL-1. eco-scale score green analytical procedure index confirmed greenness profile method through 88 has criteria,